§16§§§§Tissue engineering and regenerative medicine is a new, interdisciplinary branch of science that combines knowledge from numerous scientific fields including biology, biochemistry, physics, chemistry, applied engineering, and medicine. It aims to restore damaged parts of the human body by rebuilding them in vitro using individual building blocks of biological tissues such as cells and the extracellular matrix that surrounds them. The authors hope to spark students' interest in this exciting new field of science as well as give them a basic knowledge of its terminology.§§This book is based on a hands-on practical course in tissue engineering conducted by the Fulbright US Scholar recipient, Dr. Narine Sarvazyan (George Washington University, Washington USA). It provides an overview of the core topics of the tissue engineering field, including stem cell differentiation, the role of extracellular matrix and attachment proteins, scaffolds, and culturing of engineered t §16§issues. Each chapter is accompanied by hands-on demonstrations and self-check questions. The text is easily readable for students of all backgrounds and the described protocols can be conducted using common lab equipment. This textbook is also useful for developing undergraduate and graduate courses that teach basic methods and approaches in this promising and rapidly developing field.§§ §§ §04§§Course introduction§§Chapter 1. Reference search, image and data analysis§§Artem Oganesyan 〓§Chapter 2. Organs and tissues§§Mariam Grigoryan 〓§Chapter 3. Cells as tissue building blocks §§Anna Simonyan 〓§Chapter 4. Cell culture §§Astghik Karapetyan 〓§Chapter 5. Cell viability assays§§Hovhannes Arestakesyan 〓§Chapter 6. Extracellular matrix and attachment proteins §§Liana Hayrapetyan 〓§Chapter 7. Stems cells §§Zaruhi Karabekian 〓§Chapter 8. Imaging, staining and markers§§Vardan Avetisyan 〓§Chapter 9. Decellularization§§Narine Sarvazyan §§Chapter 10. Scaffolds. 3D printing. §§Vahan Grigorian 〓§Chapter 11. Culturing 3D engineered tissue. Bioreactors§§Arman Simonyan 〓§ §04§Chapter 12. Reporting results§ §02§ as teaching assistants. This book is an outcome of this course. Seven out of twelve students who took the course contributed to specific chapters of this book. Hasmik Mikaelyan, regional coordinator of the Fulbright program in Armenia, and Dr. Naira Ayvazian, Director of the Orbeli Institute of Physiology, are gratefully acknowledged for their support and encouragement throughout these activities.§
